Anjana Sukhani put her academic pursuits behind to pursue acting. During the early years of her career, she was cast in a television advertisement for Cadbury Dairy Milk chocolates with the Bollywood superstar Amitabh Bachchan. She was also noted for her performance in the remixed Hindi music video for the song Ghar Jayegi. Even though she does not have a film industry related background, she has figured in sizeable roles like in the 2007 multi-starrer blockbuster film Salaam-e-Ishq, following which she starred in Golmaal Returns, a sequel to the 2006 hit film Golmaal. Her other releases were Jai Veeru, Jashnn and her Kannada debut film Maleyali Jotheyali alongside Ganesh and Yuvika Chaudhary. She has been cast in Tollywood actor Ravi Teja's film Don Seenumarking her entry into tollywood.